~ 031808 Curcumin Inhibits Herpes Simplex Virus

Curcumin inhibits herpes simplex virus immediate-early gene expression by a mechanism independent of p300/CBP histone acetyltransferase activity.

Abstract

Curcumin, a phenolic compound from the curry spice turmeric, exhibits a wide range of activities in eukaryotic cells, including antiviral effects that are at present incompletely characterized. Curcumin is known to inhibit the histone acetyltransferase activity of the transcriptional coactivator proteins p300 and CBP, which are recruited to the immediate early (IE) gene promoters of herpes simplex virus type 1 (HSV-1) by the viral transactivator protein VP16. We tested the hypothesis that curcumin, by inhibiting these coactivators, would block viral infection and gene expression. In cell culture assays, curcumin significantly decreased HSV-1 infectivity and IE gene expression.

Entry of viral DNA to the host cell nucleus and binding of VP16 to IE gene promoters was not affected by curcumin, but recruitment of RNA polymerase II to those promoters was significantly diminished. However, these effects were observed using lower curcumin concentrations than those required to substantially inhibit global H3 acetylation. No changes were observed in histone H3 occupancy or acetylation at viral IE gene promoters. Furthermore, p300 and CBP recruitment to IE gene promoters was not affected by the presence of curcumin. Finally, disruption of p300 expression using a short hairpin RNA did not affect viral IE gene expression. These results suggest that curcumin affects VP16-mediated recruitment of RNA polymerase II to IE gene promoters by a mechanism independent of p300/CBP histone acetyltransferase activity.

Sebla B. Kutluaya, d, James Doroghazib, 1, Martha E. Roemerd and Steven J. Triezenberga, c, d, Corresponding Author Contact Information

a Graduate Program in Cell and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA

b Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA

c Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA

d Van Andel Research Institute, Grand Rapids, MI 49503, USA Received 3 April 2007; revised 2 May 2007; accepted 28 November 2007. Available online 14 January 2008.
Related Products and Information

Black Cumin Seed Oil with BCM-95 Bio-Curcumin - Life Extension - 60 Softgels
Retail Price: $32.00
Your Price: $24.00
LEF-01710
Super Bio-Curcumin (400 mg) - Life Extension - 60 Vegetarian Capsules
Retail Price: $38.00
Your Price: $28.25
LEF-00407
Super Bio-Curcumin - Life Extension - 4-Pak
Retail Price: $140.00
Your Price: $106.00
LEF-00407x4
Select: 
Black Cumin Seed Oil with Bio-Curcumin - Life Extension - 4-Pak
Retail Price: $120.00
Your Price: $90.00
LEF-01710x4
Select: 

Free Shipping in the Continental U.S. on Orders over $50
The statements made here have not been evaluated by the FDA. The foregoing statements are based upon sound and reliable studies, and are meant for informational purposes. Consult with your medical practitioner to determine the underlying cause of your symptoms. Please always check your purchase for possible allergins and correct dosage on the bottle before use.

While we work to ensure that product information is correct, on occasion manufacturers may alter their ingredient lists. Actual product packaging and materials may contain more and/or different information than that shown on our Web site. We recommend that you do not solely rely on the information presented and that you always read labels, warnings, and directions before using or consuming a product. For additional information about a product, please contact the manufacturer. Content on this site is for reference purposes and is not intended to substitute for advice given by a physician, pharmacist, or other licensed health-care professional. You should not use this information as self-diagnosis or for treating a health problem or disease. Contact your health-care provider immediately if you suspect that you have a medical problem. Information and statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition. Life Ex Online assumes no liability for inaccuracies or misstatements about products.